This kit is used for in vitro qualitative detection of SARS-CoV-2 and Influenza A, Influenza B in human nasal swab samples.

The assay is based on isothermal amplification method and enzyme digestion probe technology, and conserved regions are selected for specific primers and specific probes design. A large number of target sequence’s copies were generated in the reaction system during the isothermal amplification. When the probe hybridizes to the complementary sequence, it is cleaved, and fluorescence is emitted. Integrated Nucleic Acid Testing Device detects and analyzes fluorescence signal automatically, reporting negative, positive or invalid result. The assay includes internal control for monitoring of sample collection, processing, and amplification to reduce false negative results.

1. Technical introduction
1.1 Microfluidics technology: Microfluidics refers to the science and technology involved in systems that microtubes (tens to hundreds of micrometers in size) to process or manipulate tiny volumes of fluids (ranging from nanoliters to attoliters). It is an emerging interdisciplinary discipline involving chemistry, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Because of the characteristics of miniaturization and integration, microfluidic devices are commonly referred to as microfluidic chips, and also known as lab-on-a-chip devices and micro total analysis systems. 1.2 Isothermal nucleic acid amplification technology: this is the general term for a class of molecular biology technology that expands the copy number of a particular DNA or RNA fragment at a particular temperature. At present, the main isothermal amplification technologies include rolling circle nucleic acid amplification, loop-mediated is temperature amplification, strand displacement amplification, nucleic acid sequence-based amplification and helicase-dependent amplification. They all share common characteristics: constant temperature, high efficiency, special, no need for special

2. Main uses and scope of application
It can be used with adaptive microfluidic chips for in vitro amplification detection of sample nucleic acid.